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. 2016 Apr 4;7(19):28040–28058. doi: 10.18632/oncotarget.8575

Figure 6. Endothelial changes in the hippocampus 6 months after irradiation at 10D and 10W.

Figure 6

A. Representative dot-plot of hippocampus single cell suspension CD31+/ZO+ cells are gated. B. Significant increase in the number of endothelial cells in 10D-irradiated mice. C. No significant changes in the number of endothelial cells in 10W-irradiated mice. D. Representative IHC image stained with antibody against CD31 for visualization of blood vessels. Image, 10x magnification, scale bar = 100 μm; high magnification 40x, scale bar 10 μm. E. and F. Quantification of microvessel total area in the ML (dashed line), showing no significant changes in 10D-irradiated mice (P = 0.08), as opposed to a significant decreases in 10W-irradiated mice (both at 0.1 and 2 Gy). Data are presented as mean ± SEM. *P < 0.05 or **P < 0.01 for comparison with controls. The number of animals used is n = 4–5.