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. 2016 Apr 4;7(19):28075–28085. doi: 10.18632/oncotarget.8576

Figure 1. miR-218-5p directly downregulates EGFR in NSCLC cells.

Figure 1

A. Schematic representation of the putative miR-218-5p binding sequence on the 3′-UTR of the EGFR mRNA. B. The luciferase reporter plasmid encoding the full-length 3′-UTR of EGFR was co-transfected into the A549 and H1975 cells along with miR-218-5p mimic or scrambled mimic. The luciferase activities were normalized to the β-galactosidase levels of the control (**P < 0.01). C. Quantitative RT-PCR analyses of the expression levels of miR-218-5p in the A549 and H1975 cells transfected with miR-218-5p mimic, scrambled mimic, antisense miR-218-5p or scrambled antisense miRNA. The results were normalized to U6 (**P < 0.01). D. Quantitative RT-PCR analyses of the expression levels of EGFR mRNA in the A549 and H1975 cells after transfection. The results were normalized to GAPDH. E and F. Western blotting analyses of the expression levels of EGFR protein in the A549 and H1975 cells after transfection. Upper panel: representative images; lower panel: quantitative analysis (**P < 0.01).