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. 2016 Apr 5;7(19):28195–28206. doi: 10.18632/oncotarget.8618

Figure 1. Initial screening of miRNAs related to MGMT expression in glioblastoma ex vivo.

Figure 1

MGMT mRNA was quantified in FineFix–fixed and paraffin-embedded 57 samples of glioblastoma (WHO grade IV). MGMT gene promoter was non-methylated in all samples. MGMT mRNA relative expression normalized over GAPDH mRNA was analyzed by RT-qPCR with TaqMan probes. (A) Relative expression was ordered in a rank in order to select samples at the extremes of low (L) and high (H) MGMT mRNA expression. (B) Expression pattern of five miRNAs differentially expressed between MGMT-low and MGMT-high glioblastoma samples (WHO grade IV), displaying extreme levels of MGMT mRNA. MGMT gene promoter was non-methylated in all samples. (C) MiR-181 family expression in the same low- and high-MGMT glioblastoma samples.