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. 2016 Apr 7;7(19):28435–28447. doi: 10.18632/oncotarget.8633

Figure 6. IL-6 mediates the effect of downregulating COX2 on Tsc2-null cell growth.

Figure 6

A and B. Tsc2+/+, Tsc2−/− and rapamycin (Rapa)-treated (10 nM for 24 h) Tsc2−/− MEFs. C and D. ELT3 cells were treated for 24 h with or without 10 nM rapamycin (Rapa). E-H. LV- or LV-COX2-expressing Tsc2−/− MEFs (E and F) or ELT3 cells (G and H) were transfected for 48 h with IL-6 siRNAs or control siRNA (siNC). I and J. Tsc2+/+-shCOX2−2 MEFs were infected with LV or LV-IL-6. A, C, E, G, and I. qRT-PCR analysis of IL-6 mRNA levels in the indicated cells. B, D, F, H, and J. Cell supernatants from the indicated cells were collected, and IL-6 levels were determined using an ELISA. K-M. Proliferation of the indicated cells was examined using MTT assays. Error bars indicate mean ± SD of triplicate samples. **P<0.01; ***P<0.001.