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. 2016 Apr 11;7(19):28540–28555. doi: 10.18632/oncotarget.8677

Figure 3. Reduced expression of PRSS8 was associated with hypermethylation in PRSS8 promoter region.

Figure 3

A. A CpG island was identified in PRSS8 promoter region (−4116 to - 4238). B. Methylation specific PCR and PCR product sequence showed hypermethylation in 4 esophageal squamous cell carcinomas (Clones-226, -361, -985 and -505) and in 2 esophageal cancer cell lines (KYSE450, EC9706), other 2 esophageal cancer cell lines (TE1 and TE8) did not show hypermethylation. C. PRSS8 promoter truncated mutations were constructed into pGL4 vector, respectively. D. and E. PRSS8 truncated mutations showed different activities in HEK293 cells, suggesting that methylated promoter from KYSE450 cells impaired reporter activity, compared to the unmethylated promoter from the TE1 cells. The experiments were conducted three times independently.