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. 2016 Apr 16;7(19):28670–28683. doi: 10.18632/oncotarget.8764

Figure 3. FGFR2 depletion decreases CD44 and CD44 depletion decreases FGFR2 via c-Myc.

Figure 3

A. A shRNA s1-mediated transient KD of CD44 decreased FGFR2, HER2, EpCAM, and Thy1 (CD90). * denotes a significant decrease. Figure S5 shows data from multiple independent experimental replicates. B. CD44 KD decreased c-Myc and FGFR2. C. Induced CD44 KD by doxycycline (Dox) for 1 day in SNU-16-TetR-shCD44 2 cells resulted in significant decreases in Sox2 and c-Myc mRNA levels but had less profound or delayed decreases on FGFR2 mRNA, indicating that CD44 regulation of FGFR2 occurs at the posttranscriptional level rather than at transcriptional level. D. A shRNA s1-mediated transient FGFR2 KD significantly decreased CD44 and EpCAM, but Her2 and Thy1 levels were not as significantly decreased (See Figure S5 for additional data). E. FGFR2 KD resulted in increased c-Myc and decreased CD44. F. Induced FGFR2 KD for 3 days by Dox in SNU-16-TetR-shFGFR2 set 2 was accompanied by sharp increases in Sox2 and c-Myc mRNA levels but less profound or delayed CD44 mRNA decreases. The mRNA levels were determined by RT-qPCR.