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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Cell Rep. 2013 Jun 20;3(6):2075–2087. doi: 10.1016/j.celrep.2013.05.029

Figure 3. SOX2 Deletion in Primary BM-MSCs Leads to Decreased YAP1 and Reduced Colony Formation that Can Be Rescued by YAP1.

Figure 3

(A) Western analysis of SOX2, YAP1, and TAZ in BM-MSCs isolated from WT or SOX2 CKO mice.

(B) Colony assay (in fibroblast colony-forming units [cfu-f]) of BM-MSCs isolated from 4-week-old SOX2EGFP/+, osterix-CRE (heterozygous) or SOX2EGFP/F, osterix-CRE (SOX2 CKO) mice; 105 cells were plated in triplicate and analyzed as in Figure 2E. *p < 0.05.

(C) Western analysis and colony assay of SOX2F/+ or SOX2F/F BM-MSCs following in vitro CRE-mediated deletion of endogenous SOX2. Primary BM-MSCs were isolated from 4-week-old mice infected with control (GFP) or SOX2-deleting (CRE) virus. *p < 0.05.

(D) Rescue of the colony-forming ability of SOX2-deleted BM-MSCs by YAP1. Western analysis and colony assay were conducted on SOX2F/− BM-MSCs overexpressing YAP1 and depleted of endogenous SOX2 by CRE lentivirus infection.

*p < 0.05; error bars represent the average + SD. See also Figure S3.