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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Cell Rep. 2013 Jun 20;3(6):2075–2087. doi: 10.1016/j.celrep.2013.05.029

Figure 6. Moderate YAP1 Expression Enables Adipogenesis and PPARγ Expression.

Figure 6

(A) Western analysis of YAP1 expression. C3H10T1/2 cells were infected with YAP1, YAP1shRNA (shYAP1), or shYAP1 and YAP1 (shYAP1 +YAP1 rescue) lentivirus.

(B and D) Adipogenesis and osteogenesis in cells expressing different levels of YAP1. Cells were transduced as in (A), incubated in adipogenic (B) or osteogenic (D) media for 10 days, and stained as in Figures 4A and 4B.

(C) PPARγ expression is regulated by YAP1 during adipogenesis. Western analysis of C3H10T1/2 cells expressing YAP1 or shYAP1 during adipogenic differentiation for the indicated days is shown.

(E) YAP1 is a key downstream target of SOX2 in osteogenesis. Osteogenesis in C3H10T1/2 cells expressing control or SOX2 lentivirus that were infected with either control (scrambled) or shYAP1 is shown.

(F) Adipogenesis assay of cells infected as in (E).

(G) Western analysis of cells used in (E) and (F). See also Figure S6.