Table 1.
[a] |
[b] |
[c] |
[d] |
[e] |
[f] |
[g] |
[h] |
[i] |
---|---|---|---|---|---|---|---|---|
Chimera type |
GFP ESC cell line |
Recipient type |
Total embryo #'s transferred (# of trials) |
Total implantation sites [IP] (% of ET) |
# Normal fetuses recovered (% of total IP) |
# Abnormal fetuses recovered (% of total IP) |
# of reabsorbed/ empty sacs (% of total IP) |
# GFP, YFP or bang particle normal fetuses (% of total IP) |
Mouse 2N blastocyst- injection |
Rhesus nhp2706 |
ICR | 147 (9) | 56 (38) | 43 (77) | 8 (14) | 5 (9) | 0 |
Rhesus nhp106 |
ICR | 60 (3) | 25 (42) | 25 (100) | 0 | 0 | 0 | |
Baboon ESC-4 |
NOD-SCID | 32 (4) | 8 (25) | 5 (63) | 0 | 3 (38) | 0 a | |
Mouse ESC- YFP |
ICR | 48 (4) | 16 (33) | 12 (75) | 0 | 4 (25) | 5 (31) | |
Mouse 2N embryo- aggregation |
Rhesus nhp2706 |
ICR | 39 (4) | 37 (95) | 7 (19) | 19 (51) | 11 (30) | 0 b |
Rhesus nhp 3006 |
ICR | 18 (1) | 18 (100) | 2 (11) | 6 (33) | 10 (56) | 0 b | |
Mouse ESC- YFP ± bang particles |
ICR | 42 (4) | 14 (33) | 6 (43) | 0 | 8 (57) | 5 (36) | |
Mouse 4N- injection |
Rhesus nhp2706 |
ICR | 9 (1) | 9 (100) | 0 | 0 | 9 (100) | 0 c |
Rhesus nhp106 |
ICR | 7 (1) | 0 | 0 | 0 | 0 | 0 | |
Rhesus nhp3006 |
ICR | 7 (1) | 0 | 0 | 0 | 0 | 0 |
2/4 recipients died prior to fetal analysis on day E12 post transfer. 1 reabsorbing embryo expressed nhpESC-GFP cells.
9/25 abnormal fetal tissues demonstrated surviving nhpESC-GFP cells.
5/9 reabsorbing or empty sacs demonstrated surviving nhpESC-GFP cells.