Table 1.
Levels (pg/mg) of cytokines, chemokines, and growth factors in the testes homogenates following CNC exposure.
| Control | CNC | |
|---|---|---|
| IL-1α | 1.14 ± 0.03 | 1.28 ± 0.07* |
| IL-1β | 10.38 ± 0.51 | 12.15 ± 0.35* |
| IL-2 | 2.23 ± 0.10 | 2.74 ± 0.20* |
| IL-3 | ND | ND |
| IL-4 | ND | ND |
| IL-5 | 1.09 ± 0.07 | 1.25 ± 0.08 |
| IL6 | 0.05 ± 0.02 | 0.06 ± 0.02 |
| IL-9 | ND | ND |
| IL-10 | 5.06 ± 0.21 | 5.39 ± 0.17 |
| IL-12p40 | 2.41 ± 0.11 | 2.56 ± 0.17 |
| IL-12p70 | 3.63 ± 0.31 | 9.12 ± 1.19* |
| IL-13 | 44.60 ± 3.87 | 63.28 ± 3.23* |
| IL-17A | 3.07 ± 0.23 | 3.35 ± 0.17 |
| Eotaxin | 94.25 ± 8.41 | 120.52 ± 15.09 |
| G-CSF | 3.10 ± 0.68 | 3.24 ± 0.74 |
| GM-CSF | ND | ND |
| IFN-γ | 0.06 ± 0.01 | 0.09 ± 0.02 |
| KC | 1.68 ± 0.07 | 2.63 ± 0.28* |
| MCP-1 | 9.39 ± 0.42 | 11.48 ± 0.35* |
| MIP-1α | 0.46 ± 0.03 | 0.58 ± 0.06 |
| MIP-1β | 4.49 ± 0.09 | 5.14 ± 0.27 |
| RANTES | 0.80 ± 0.05 | 1.64 ± 0.15* |
| TNF-α | 11.88 ± 0.33 | 16.43 ± 0.95* |
Note. Mice were exposed twice a week for 3 consecutive weeks with a cumulative dose of 240 μg/mouse of CNC and sacrificed 3 mo following their last exposure. Each measurement was assayed in duplicate and normalized by total protein content in testes homogenates. Values are means ± SE (n = 10 mice per group). Asterisk indicates significantly different from controls (p < .05). ND corresponds to levels that were below detection limits of the kit.