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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: FEBS J. 2016 Oct;283(19):3567–3586. doi: 10.1111/febs.13819

Table 1.

Thermal stability of UbaA and its variants determined by differential scanning fluorimetry (DSF) in the presence and absence of nucleotides.

UbaA Variant Ligand Tm
(°C)
Tm Shift
(°C)
wt -a 58.60 ± 1.13 -
ATP 66.80 ± 3.96 8.20 ± 4.12
AMP 59.90 ± 0.42 1.30 ± 1.21
ADP 63.00 ± 0.85 4.40 ± 1.41
AMP-PNP 66.00 ± 3.39 7.40 ± 3.58
CTP 57.60 ± 1.13 -1.00 ± −1.60
GTP 59.60 ± 1.41 1.00 ± 1.81
TTP 59.70 ± 2.12 1.10 ± 2.40
UTP 59.70 ± 0.99 1.10 ± 1.50

K87R - 59.68 ± 1.42 -
ATP 60.12 ± 0.46 0.44 ± 1.61

D131N - 61.12 ± 1.85 -
ATP 60.92 ± 3.02 −0.20 ± 3.54

C188A - 56.08 ± 2.04 -
ATP 63.20 ± 1.11 7.12 ± 2.32
a

-, no ligand control. Means of thermal shift difference and standard deviations were calculated (n = 2 for the nucleotide ligands, n = 6 for the UbaA variants). See Methods for assay details.