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. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: Vaccine. 2016 Sep 9;34(44):5336–5343. doi: 10.1016/j.vaccine.2016.08.078

FIG. 4. Kinetic analysis of Th1 and Th17 cell types correlated with vaccine-induced protection.

FIG. 4

(A) Percentages of Th1 (IFN-γ+) and Th17 (IL-17A+) cells within the gated Th cells (CD4+CD8−) population in the lungs of naïve, non-vaccinated (Ctl.) versus vaccinated (ΔT) mice were shown at 7 and 9 dpc. As an extension of this analysis, the percentages of these two T-cell subsets were examined in fetal acute disease (FAD) group at 14 dpc and in disseminated (DD) and pulmonary disease (PD) groups at 14, 35, and 50 dpc (B). Numbers left to the gates indicate the mean percentages of the gated Th1 and Th17 subsets. (C, D) The cell numbers of gated Th1 and Th17 per lungs were determined by intracellular cytokine staining and mean cell values ± SEM were plotted for each group of mice at 7, 9, 14, 35 and 50 dpc. Asterisks indicate significant differences in cell numbers between non-vaccinated (Ctl.) and ΔT-vaccinated groups at 7 or 9 dpc (Mann-Whitney U test; P < 0.05). The single dagger indicates significant differences between the PD and the DD groups (†, P < 0.05), exclamation mark indicates significant differences between the FAD and the DD groups (!, P < 0.05), while the double dagger indicates significant differences between acute and pulmonary disease groups (‡, P < 0.05) at 14, 35, or 50 d. The data presented are representative of two independent experiments.