Table 4. AIV detection using RT-LAMP-microarray and one step RT-PCR for field samplesa).
RT-LAMP-microarray assay | |||
---|---|---|---|
H6 N1+ | H5N2 + | Novel H5 + | |
One step RT-PCR | |||
H6N1 + | 7 | 0 | 0 |
H6N1 − | 7 | 0 | 0 |
H5N2 + | 0 | 12 b) | 0 |
H5N2 − | 0 | 0 | 0 |
Novel H5 + | 0 | 0 | 15 |
Novel H5 − | 0 | 0 | 1 |
a) Forty-two samples showed AIV RT-LAMP-microarray positive among the total 72 field samples. b) Eleven showed H5 LAPIV (PQREKR*GLF), and one showed H5 HPAIV (PQRKKR*GLF/PQRRKR*GLF) within these 12 samples.