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. 2016 Apr 21;78(8):1237–1244. doi: 10.1292/jvms.15-0688

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©2016 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 5. — The immunohistochemical features of canine TZL. (a) Neoplastic T cells in the paracortex are uniformly immunopositive for CD3. Immunolabeling with anti-CD3. × 40. (b) Intense labeling of neoplastic T cells with CD3. The neoplastic T cells are small or intermediate in size. Immunolabeling with anti-CD3. × 1,000. (c) B cells in fading lymphoid follicles are immunopositive for CD20. Follicular structures are pressed against the outer sinus by the expanding population of neoplastic T cells. Immunolabeling with anti-CD20. × 40. (d) Neoplastic cells in the paracortex are strongly immunopositive for CD25. B cells in the lymphoid follicles are negative for CD25. Immunolabeling with anti-CD25. × 40. (e) CD25-positive neoplastic cells are small to medium in size. Some cells have sharp shallow nuclear indentations and abundant cytoplasm. Immunolabeling with anti-CD25. × 1,000.