. 2016 Apr 21;78(8):1237–1244. doi: 10.1292/jvms.15-0688

Table 1. Primers used for the detection of clonal rearrangement of IgH and TCRγ genes^a).

Reaction number	Product	Primer specificity	Primer sequence (5′ −3′)^b)
1	Cµ	Cµ	TTC CCC CTC ATC ACC TGT GA
		Cµ	GGT TGT TGA TTG CAC TGA GG
2	IgH major	VH	CAG CCT GAG AGC CGA GGA CAC
		JH	TGA GGA GAC GGT GAC CAG GGT
3	IgH minor	VH	CAG CCT GAG AGC CGA GGA CAC
		JH	TGA GGA CAC AAA GAG TGA GG
4	TCRγ	JH	ACC CTG AGA ATT GTG CCA GG
		JH	GTT ACT ATA AAC CTG GTA AC
		VH	TCT GGG A/GTG TAC/T TAC TGT GCT GTC TGG
5	IgH	VH	GMC GVT TCA CCA TCT CCA RRG
		JH	TGA RGA GAC RGT GAC CWG GGT
		JH	GGA CAC GAA GAS TGA GGT GCC
6	TCRγ	Vγ	TGK TGC AGA ARC TGG AGA AGA
		Jγ	GCA CTG TGC CAG GAC CAA ATA

a) IgH: immunoglobulin heavy chain, TCRγ: T-cell receptor γ chain. b) K: guanine (G) + thymine (T), R: adenine (A) + G, M: A + cytosine (C), W: A + T, S: C + G, V: A + C + G. Reaction Numbers 1−4: Primers reported by Burnett et al. [4], Reaction Numbers 5 −6: Primers reported by Valli et al. [27]

Table 1. Primers used for the detection of clonal rearrangement of IgH and TCRγ genesa).

Table 1. Primers used for the detection of clonal rearrangement of IgH and TCRγ genes^a).