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Determination of bacterial counts in heart, liver, spleen, lung and kidney. The mice were infected with 2 × 106 CFU of the WT ExPEC strain PPECC42, ompX mutant and complemented strain. Aliquots (0.1 g) of tissues were homogenized, serially diluted and spread on TSA plates to determine bacterial counts. All values of the bar graphs are presented as the mean ± SD. Asterisks indicate significant differences between the values of the mutants and the WT strain (*P<0.05; **P<0.01).
