Figure 5.

Staining of neurons contributing to terminals in the bulbs. (A) Immunostaining for γ-aminobutyric acid (GABA; green) using the antibody from Abcam. GABA-immunoreactive TL neurons branch in the LBUs and the larger MBUs. The neurons have dense ramifications in the CBL whereas the upper division (CBU) is barely stained. (B,C) Double labeling by injection of dextran Texas Red into the AOTU, labeling TuLAL1 neurons (magenta) and immunostaining for GABA (green) with the antibody of Kingan, labeling TL neurons, reveals the structure of microglomerular synaptic complexes. (B) In the MBU TL3 neurons form complexes with large terminals of TuLAL1b neurons. The TuLAL1b neuron terminals are located on top of the TL neuron branches. (C) In the LBU the complexes have a similar structure. Here, GABA immunostaining exposes a bushy structure at the tip of the extension of a TL neuron (arrowhead). Scale bars: A = 50 μm, B,C = 10 μm.