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. 2016 Oct 7;6:118. doi: 10.3389/fcimb.2016.00118

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Copyright © 2016 Huang, Ma, Shang, Hu, Liang, Li, Wu, Dai, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagrams of three groups of ICEs inserted at rplL and rum loci. Int, relaxase, VirB4, and AMR determinants were indicated in blue, light blue, red, and green, respectively. MGEs integrated were highlighted in black straight line. ORFs identity was shown in light gray shadow and identity less than 90% were indicated. (A) Comparisons of Tn5252 groups. The prototype ICE Tn5253 sequence was retrieved from NCBI under accession EU351020. Tn5253 integrated at rbgA site. (B) Comparisons of ICESp1108 group. This group of ICEs was previously classified into Tn1549 group, but this study showed different genetic context thus was classified as ICESp1108 group. (C) Comparisons of TnGBS2 group. ICESsuTYPE3 is highly identical with ICESp1116 in core structure, a TnGBS2 family ICEs.