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. 2016 Oct 7;7:408. doi: 10.3389/fimmu.2016.00408

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Copyright © 2016 van Beek, Sovran, Hugenholtz, Meijer, Hoogerland, Mihailova, van der Ploeg, Belzer, Boekschoten, Hoeijmakers, Vermeij, de Vos, Wells, Leenen, Nicoletti, Hendriks and Savelkoul.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of B cells and T cells in Peyer’s patches (PP) and mesenteric lymph nodes (MLN) upon bacterial supplementation in Ercc1^−/Δ7 mice. (A,B) Mean frequencies of B and T cells in PP and MLN were determined by flow cytometry. B cells were defined as CD19⁺, T cells were defined as CD3⁺. (C) Flow cytometric analysis of CD3⁺CD4⁺CD8⁻ regulatory T (Treg) cells in MLN. (D) Mean frequencies of Treg cells in MLN. Data represent the mean + SEM from four to six animals per group. LP, L. plantarum WCFS1; LC, L. casei BL23; BB, B. breve DSM20213. *p < 0.05; **p < 0.01.