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. 2016 Oct 7;10:226. doi: 10.3389/fncel.2016.00226

Figure 2.

Figure 2

Increased translation of ATXN2, ATXN3, and ATXN7 constructs with expanded CAG repeats in vitro. (A) Schematic showing luciferase reporter constructs used in this experiment. Luciferase reporter constructs were cloned that contain the CAG repeat region of ATXN2, ATXN3, and ATXN7 in the 3′UTR. Two constructs each with either normal (left) or mutant CAG (right) repeat lengths were cloned. (B) In vitro translation of the luciferase reporters of ATXN3. Previously published HTT exon1 luciferase reporter constructs were included as positive controls (Krauss et al., 2013). The constructs with either normal or mutant CAG repeat lengths were in vitro transcribed and equimolar amounts of RNA were subjected to in vitro translation. The level of translated luciferase reporter was measured in a luciferase assay. Columns represent mean values ± SEM. n = 3, p ≤ 0.01. (C) In vitro translation of the luciferase reporters of ATXN2 and ATXN7. The constructs with either normal or mutant CAG repeat lengths were in vitro transcribed and equimolar amounts of RNA were subjected to in vitro translation. The level of translated luciferase reporter was measured in a luciferase assay. As a negative control, a sample without RNA was included. Columns represent mean values ± SEM. n = 3, p ≤ 0.0001. *Indicates statistically significant differences (p < 0.05).