Figure 3.

Neutral lipid accumulation in Mycobacterium marinum-infected D. discoideum. (A) Prior to infection, amoebae are incubated with palmitic acid, corresponding to the High Fat Diet (HFD). This triggers the rapid formation of LB which leads to a foamy appearance. (B) Within 10 min post-infection with M. marinum, LB are gathered around the mycobacteria-containing vacuoles. (C) Infection with amikacin-killed mycobacteria fails to induce LB movement/relocation to the mycobacteria-containing vacuoles. (D) At 3 h post-infection, intact LB are found within the lumen of M. marinum—containing vacuoles. At this stage, a few ILI are already detectable inside the bacteria. (E) At later stages (19 h post-infection), neutral lipids are homogenously distributed inside the mycobacterial cytosol where they accumulate in the form of large ILI.