Fig. 1.

The optimal number of technical replicates for measurement evaluation experiments. The graphs show the dependence of the variance of the ratio of technical variance to the total variance (Variance) on the number of technical replicates (Tech Rep) and the relative contribution of the biological variability [Rho squared (ρ²)]. The total number of available measurements (M) is fixed in both cases as M = 30. A. Visualization of the analytical solution. B. Results of 10,000 times of simulations.