Fig. 9.

The initial (C1) and combined (C2) clusters that demonstrate higher expression under nitrogen limitation (A). The normalized expression profiles are shown in red (high) and green (low). For each gene it is indicated if either of the four known nitrogen related motifs (DAL82, GAT1, GLN3, GZF3) is present in its upstream region (B). The GO categories that show the highest enrichment in C1 and C2 are Catabolism (GO1) and Allantoin Catabolism (GO2), and the genes annotated with the two categories are denoted (C). The column “Both” indicates the genes that are found in both C1 and C2 (D). In the second cluster, only the genes with a clear regulation remain. In this way, we have gained more confidence in the co-regulation of this cluster.