Figure 3.

Negative impact of UbiC on U6 promoter activity enhanced in the presence of CMV enhancer. Legends in Figure 2 was followed except that CMV enhancer was placed between U6 and UbiC promoter or fused to the upstream of U6 as plotted. A. Diagram of U6 promoter, CMV enhancer, and UbiC promoter arrangements. CMV enhancer was fused to the upstream of either U6 promoter or UbiC promoter forming divergent or tandem arrangements as indicated. B. Western blot of mCIN85 to reflect the residual mCIN85 left in the cells. Samples were generated using different virus transduction as indicated. C. Relative U6 promoter activity after normalization against α-tubulin. Values are statistics from three independent experiments.