Figure 5.

A. Amino acid sequence alignment of oryzacystatin (OC1) and wheat cystatins (WCs). The positions of secondary structures of OC1 are indicated except the first β-strand, which is not shared by most of the WCs. An array of red ovals indicates an α-helix and block arrows indicate β-strands. α-helices of WCs are shown in yellow boxes and β-strands are in cyan boxes. The four signature sequences of PhyCys are shown enclosed in differently colored rectangles. B. Diagrammatic representation of secondary structures of different wheat cystatins. Structure of OC1 is shown for comparison. Structures are not up to scale.