Figure 1.

Comparison of staining using four silver staining protocols. Proteins extracted from young rice caryopsis were loaded as sample and SDS-PAGE was carried out at 10 mA/gel for 15 min followed by 40 mA/gel for 3 h until the dye front just ran off the edge of the gel. The gel was cut into four pieces equally, which were stained using four sliver staining protocols (referred as A, B, C, D) respectively and shown in panel A, B, C, D, respectively. Lane M1 and M2 were loaded with the standard protein marker; lanes 1-4 were loaded with 2 µg of sample; lanes 5-8 were loaded with 4 µg of sample. These experiments were repeated four times.