Comparison of staining using four CBB staining protocols. Protein samples were loaded and separated similarly as indicated in Figure 1. SDS-PAGE gel was cut into 4 equal pieces, which were stained using four CBB staining protocols (referred as A, B, C, D) respectively and shown in panel A, B, C, D, respectively. Lane M indicated the standard protein marker; lane 1-7 was loaded with 35, 30, 25, 20, 15, 10 and 5 µg of sample, respectively. These experiments were repeated four times.