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Comparison of separation of 2-DE using IPG strips with pH 4-7 and pH 5-8 to IEF. Total proteins were extracted from young rice caryopsis on the 10th day after heading and 120 µg of protein was loaded as sample. 2-DE was carried out using 17 cm-long nonlinear IPG strips with pH 4-7 (left panel) and pH 5-8 (right panel) to IEF, respectively. These experiments were repeated four times.
