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Evaluation of sample loading quantity for 2-DE. Proteins extracted from young rice caryopsis were loaded with various quantities including 100 µg (A), 115 µg (B), 130 µg (C) and 145 µg (D) as indicated, and 2-DE was carried out using 17 cm-long nonlinear IPG strips with pH 5-8 to IEF. These experiments were repeated four times.
