Table 1.
gsMapper assembly (with Bos taurus reference mRNA database) | ||
No. of reads | 101,244 | |
No. of bases | 24,127,779 | |
No. of mapped reads | 59,211 | 41.40% |
No. of mapped bases | 6389,585 | 26.48% |
No. of unmapped reads | 41,530 | 41.02% |
No. of too short reads | 503 | 0.50% |
No. of contigs | 750 | |
No. of bases | 151,663 | |
De novo assembly for unmapped and too short reads | ||
No. of contigs | 230 | |
No. of bases | 102,080 | |
No. of singleton reads | 29,273 | |
No. of singleton bases | 6503,535 |
Note: Reads available after sequencing were mapped against the Bos taurus reference mRNA database. Unmapped reads after merging with too short reads are subjected to de novo assembly to form contigs. Assembled contigs were utilized for analysis.