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. 2016 Sep 24;10:108–118. doi: 10.1016/j.redox.2016.09.011

Fig. 1.

Fig. 1.

Effects of HBO on intracellular levels of ROS and NO and their role in HBO induced HSP32 expression in spinal neurons. ROS scavenger NAC (5 mM) or NOS inhibitor L-NAME (100 μM) was added to the medium 30 min before HBO exposure. The intracellular ROS and NO were detected immediately after HBO exposure using immunofluorescence assay (A-H). The expression of HSP32 was detected by western blot 12 h after HBO exposure (I). The effects of L-NAME at higher concentrations (J). Significance compared with Air group, **p<0.01; significance compared with HBO group ††p<0.01. HBO, hyperbaric oxygen; HSP, heat shock protein; L-NAME, NG-Nitro-L-arginine methyl ester; NAC, N-Acetyl-L-cysteine; NO, nitric oxide; NOS, nitric oxide synthase; ROS, reactive oxygen species.