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. 2016 Sep 24;10:108–118. doi: 10.1016/j.redox.2016.09.011

Fig. 7.

Fig. 7.

Effects of MEK1/2 knockdown on HBO induced HSP32 expression. Neurons were transfected with vector, shMEK1 (Seq1, Seq2 and Seq3) or shMEK2 (Seq4, Seq5 and Seq6) as described in the Materials and Methods section. Transfection efficiency was confirmed by checking MEK1 and MEK2 mRNA transcription levels (A, B). The combined effects of shMEK1 (Seq 2) and shMEK2 (Seq 4) on the expression of MEK1/2 (C) and on the expression of HSP32 (D) was observed by western blot. Significance compared with Air group, **p<0.01; significance compared with HBO group, ††p<0.01.