Fig. 3.

GlcNAc-rich motifs are efficiently recognized by lectin fluorescence on adult flukes but not by lectin blots. GlcNAc- and GalNAc-rich glycoconjugates are highly expressed on FhTeg as visualized in lectin fluorescence micrographs but only a few protein bands were recognized by lectin blots of adult Fasciola hepatica tegumental coat. Flat fixed whole adult flukes were probed with fluorescein-labeled GlcNAc- and GalNAc-binding lectins (green) and, where displayed, with DAPI nuclear staining (blue). In lectin blots, tegumental proteins (15 μg) were probed with GlcNAc- and GalNAc-binding biotin-labeled lectins or IR-labeled streptavidin complex (green), molecular mass marker (red).