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Chronic activation of mTORC1 in MEFs increases the expression of JUP, ADLOA, ACO2, and MDH2 proteins. Wild-type and TSC2−/− MEFs were cultured in complete DMEM. A, After lysis, 20 μg of total protein were subjected to Western blot using the indicated antibodies. B, After lysis, total RNA was extracted and subjected to RT-qPCR analysis for Mdh2, Jup, Aco2, Aldoa, Hspd1, and Rps6. C, Quantification and summary table of panels A and B.
