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. 2016 Jul 25;15(10):3256–3269. doi: 10.1074/mcp.M116.058164

Fig. 1.

Fig. 1.

Experimental setup and characteristics of SWATH assay libraries and SWATH measurements. Comprehensive SWATH assay libraries were generated for M. extorquens and S. melonis based on various sample types and proteomic changes during growth on the leaf surface was subsequently studied by SWATH MS (A). SWATH assay library coverage of M. extorquens PA1 and S. melonis Fr1 (B). Gray shadings indicate the number of unambiguous peptide identifications per protein. Percentage of quantified and differentially regulated target proteins per strain (C). For (B) and (C), 100% corresponds to all annotated proteins within the genome. Dynamic quantification range of the two sample types analyzed by SWATH MS measurements of both strains (D). The abundance of all identified proteins was estimated using the best flyer approach (see Methods). Overview of differentially regulated proteins of M. extorquens and S. melonis during colonization of leaves or minimal media plates (E). 100% corresponds to the total number of differentially regulated proteins per strain.