Table 2. Ranked Correlation Analysis of Metabolites (M1, M2, CB) and Mean Infant (CB) DNA Methylation.
| Top 10% M1 Metabolites Correlated with CB Mean DNA Methylation (n = 32) | ||||||
|---|---|---|---|---|---|---|
| Infant Cord Blood - Mean DNA Methylation | ||||||
| Metabolite Rank | LUMA | LINE-1 | IGF2 | H19 | ESR1 | PPARα |
| 1 | C16.1* | 24.1* | 20.3* | Val* | 20.0* | 22.6* |
| 2 | 22.1* | His* | C20.4* | Iso | 22.6* | C20.0* |
| 3 | 24.1* | 24.0* | 20.4 | 14.0 | 22.5* | 24.1* |
| 4 | C10.1* | C16.1* | C10.0 | Cys | 18.3n-6* | 22.5 |
| 5 | C18.0* | Trp* | C14.0OH | Pro | His* | 18.3n-6 |
| 6 | 24.0* | C10.1* | C10.1 | Phe | 220* | Val |
| 7 | His* | 22.1* | C12.0OH | Leu | 24.1* | C18.2 |
| 8 | 22.4* | C18.0* | C16.1 | C14.0 | C10.1* | C18.1 |
| Top 10% M2 Metabolites Correlated with CB Mean DNA Methylation (n = 32) | ||||||
| 1 | 22.1* | 24.0* | 18.2* | 18.3n-6* | C10.1* | 24.1* |
| 2 | 24.0* | C10.1* | Car* | C6.0* | 22.5* | C10.0* |
| 3 | 24.1* | 22.1* | 20.3* | C14.0OH* | 22.6* | C12.0* |
| 4 | C10.1* | C16.1* | 20.4* | Trp* | 24.0* | 22.5* |
| 5 | 22.5* | 24.1* | 20.1* | C18.2OH* | 24.1* | 22.6* |
| 6 | 22.6* | 22.5* | SumFFA* | 18.0* | 20.3* | C8.0* |
| 7 | C16.1* | 22.6* | C3.0* | Pro | C12.OH* | C10.1* |
| 8 | C18.0* | Trp* | 16.1 | C5.0DC | 22.1* | C12.0OH* |
| Top 10% CB Metabolites Correlated with CB Mean DNA Methylation (n = 33) | ||||||
| 1 | 24.1*** | C18.0*** | His* | Cys* | His** | C8.1 |
| 2 | 22.1*** | 24.1*** | Trp* | C5.0 | 18.3n6** | C18.0 |
| 3 | C10.1*** | 22.5*** | 22.5 | C20.4 | C10.1** | Sar |
| 4 | 22.5*** | 24.0*** | 20.3 | C20.0 | 22.6** | C18.1 |
| 5 | 24.0*** | 22.6*** | Glu | C20.3 | 22.5* | 24.1 |
| 6 | 22.6*** | 22.1*** | Sar | 20.0 | 24.1* | C20.0 |
| 7 | C16.1*** | C10.1*** | OH-Pro | X18.3n6 | C18.0* | C20.3 |
| 8 | Val** | His*** | 24.0 | Trp | Sar* | Lys |
Kendall’s Tau, non-parametric correlation analyses comparing metabolites (M1, M2, CB) to CB DNA methylation were ranked by p-value. Significant correlations are denoted by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001. The metabolite with the most significant p-value is ranked as number 1. Bold font indicates positive correlation coefficients; standard font indicates negative correlation coefficients. SumFFA is a summation measure of the 22 free fatty acids measured in this study. To differentiate fatty acids from acylcarnitines, acylcarnitines have a ‘C’ placed in front, e.g. C10.1; whereas fatty acids begin with the number of carbons in their chain, e.g. 24.1.