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. 2016 Oct 7;6:34830. doi: 10.1038/srep34830

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Copyright © 2016, The Author(s)

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(A) Reduced autoradiograms of pulse chase labelling of flag-parkin. HeLa cells transfected with parkin WT, parkin C289G with or without DNAJB8 were pulse labeled with [35S]-labeled cysteine and methionine for 10 minutes and chased for the indicated time periods. Following detergent lysis, parkin WT and parkin C289G were immunoprecipitated with monoclonal flag antibody. Reduced samples were resolved by 10% SDS-PAGE. (B) Detergent soluble parkin WT or parkin C289G was quantified by phosphoimaging and data was plotted as a semi-log graph(n = 3 independent samples, mean ± SD).