Figure 6. Effects of URE on nerve morphological alterations induced by CCI.
URE (70 mg kg−1, p.o., daily) and mecamylamine (meca, 2.0 mg kg−1, i.p., bid) were administered starting on the day of surgery. On day 14, 24 h after the last treatment, 5 μm nerve sections of osmium fixed sciatic nerves collected 900 μm proximally to the injury were analyzed. Ligated nerves (CCI) were compared with nerve of sham-operated animals. Effect of repeated administration of URE + vehicle or URE + mecamylamine on the number of fibers, fiber diameter, axon diameter and myelin thickness of large and small fibers are shown. Quantitative analysis was performed evaluating 6 animals for each group. Each value represents the mean ± SEM of 6 rats per group, performed in 2 different experimental sets. ^P < 0.05 and ^^P < 0.01 versus sham + vehicle; *P < 0.05 and **P < 0.01 versus CCI + vehicle; °°P < 0.01 versus CCI + URE.