Table 1. Morphometric evaluations of nerve alterations at progressive distances from CCI.
| distance to ligation | Large fibers |
Small fibers |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| treatment | number of fibers | fiber diameter | axon diameter | myelin thickness | treatment | number of fibers | fiber diameter | axon diameter | myelin thickness | |
| 300 μm | sham + vehicle | 509.8 ± 23.5 | 10.67 ± 0.08 | 6.76 ± 0.03 | 1.95 ± 0.03 | sham + vehicle | 23.7 ± 4.5 | 5.10 ± 0.21 | 3.59 ± 0.23 | 0.85 ± 0.11 |
| CCI + vehicle | 273.3 ± 45.3^ | 8.05 ± 0.23^^ | 5.93 ± 0.17^^ | 1.06 ± 0.03^^ | CCI + vehicle | 217.0 ± 50.0^ | 4.80 ± 0.08 | 3.33 ± 0.18 | 0.73 ± 0.06 | |
| 900 μm | sham + vehicle | 500.8 ± 19.5 | 10.23 ± 0.1 | 6.8 ± 0.05 | 1.93 ± 0.03 | sham + vehicle | 40.5 ± 7.6 | 5.1 2 ± 0.15 | 3.61 ± 0.15 | 0.82 ± 0.09 |
| CCI + vehicle | 336.5 ± 2.0^ | 8.04 ± 0.07^^ | 5.85 ± 0.01^^ | 1.09 ± 0.01^^ | CCI + vehicle | 240.7 ± 70^ | 5.13 ± 0.86 | 2.91 ± 0.21 | 0.83 ± 0.07 | |
| 1800 μm | sham + vehicle | 505.3 ± 20.2 | 10.40 ± 0.08 | 6.70 ± 0.08 | 1.98 ± 0.05 | sham + vehicle | 33.7 ± 3.5 | 5.10 ± 0.21 | 3.57 ± 0.17 | 0.83 ± 0.10 |
| CCI + vehicle | 353.5 ± 49.5^ | 9.33 ± 0.43^^ | 6.06 ± 0.13^^ | 1.63 ± 0.14^^ | CCI + vehicle | 143.7 ± 23.7^^ | 4.88 ± 0.10 | 3.09 ± 0.08 | 0.89 ± 0.01 | |
Morphometric measurements of number of fibers, fiber diameter, axon diameter and myelin thickness on small and large fibers from sciatic nerves of sham + vehicle and CCI + vehicle (day 14 after surgery). Five μm transverse sections of osmium fixed nerves were analyzed. Each value represents the mean ± SEM of 6 rats per group, performed in 2 different experimental sets. ^P < 0.05 and ^^P < 0.01 versus sham + vehicle.