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. 2016 Oct 7;6:34793. doi: 10.1038/srep34793

Figure 1. Intracranial injection of ZIKV results in widespread infection and activation of glia cells.

Figure 1

Wild type C57BL/6 mice were injected intracranially with saline or ZIKV (MR766) at one week of age (postnatal day 7, P7), and brains were collected 4 days post-inoculation (dpi) for immunohistochemistry. (A) Graph showing percentage of initial body weight of saline or ZIKV injected mice at over 4dpi. Two-Way ANOVA revealed main effects on interaction, F(4,56) = 44.88, p < 0.001, time points, F(4,56) = 70.50, p < 0.001, treatments, F(1,14) = 7.42, p = 0.017. Sidak’s post hoc tests were used. *p < 0.05, and ***p < 0.001. N = 8 animals per group. (B) Images showing anti-ZIKV (green) and DAPI (blue) staining in saline and ZIKV injected wild type mouse brains. Scale bar: 500 μm. (C) Images showing anti-Axl (green) staining in a wild-type mouse brain at postnatal day 8. Scale bar: 500 μm. (D) Images showing anti-Iba1 (green) and anti-GFAP (red) staining in saline and ZIKV injected wild type mouse brains. Scale bar: 500 μm. (E) Higher magnification of anti-Iba1 (green) and anti-GFAP (red) staining in saline and ZIKV infected wild type mouse brains. Scale bar: 50 μm. Iba1-postive microglia exhibited morphology consistent with activation in ZIKV infected mice. Images are representative of 5-6 sections per animals from 6 animals per group (saline and ZIKV injected).