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. 2016 Oct 7;6:34794. doi: 10.1038/srep34794

Figure 5. Glutathione and N-acetylcysteine improves impaired migration activity of PMNs.

Figure 5

(a) Numbers of transmigrating PMNs of diabetic (n = 4) comparing with healthy (n = 4) individuals. Purified PMNs were added into the upper wells and co-cultured with live B. pseudomallei put into the lower wells. After co-culture for 1 h, transmigrating PMNs were counted by flow cytometry. Asterisks indicate significant differences between healthy and DM individuals at the same concentrations. (b) Purified PMNs from healthy individuals (n = 3) were treated by 50 μM glibenclamide (GB) before infection with B. pseudomallei, S. enterica serovar Typhimurium (Sal), and E. coli at MOI of 10:1. Asterisks indicate significant differences between glibenclamide treatment and medium alone in the same conditions by paired t test. (c) Purified PMNs from healthy (n = 3) and diabetic (n = 3) individuals were treated by 12.5 mM glutathione or 12.5 mM NAC before addition to the upper wells and co-cultured with B. pseudomallei at MOI of 3:1 or (d) with 40 ng/ml of recombinant human IL-8 (rIL-8). Asterisks indicate significant differences between medium control and not-treated (0) or between not-treated and other conditions. P values were calculated by using One Way ANOVA. Data are expressed as median with range, and samples were assayed in duplicate. No asterisk or ns; non significant, *P < 0.05, **P < 0.01.