Figure 4. Unexpanded ELAV-GAL4 X UASQ25 vs. expanded ELAV-GAL4 X UASQ120 expressing flies results are depicted here.

NADH emission obtained with 740 nm two-photon excitation is shown under the intensity map. Lifetime color map of NADH is depicted on the right (color map). Shorter lifetime is color coded with red here while longer lifetime on the phasor plot is associated with cyan. As it is shown here Htt 120Q cells are characterized with a distinct population shifted toward shorter lifetime (red) that has a higher ratio of free to bound NADH. Panels C&D show the phasor plot for 25Q and 120 Q respectively. The phasor histogram is shifted toward the direction of free NADH (glycolysis) in the expanded form 120Q compared to unexpanded 25Q. Scale bar is 10 um.