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. 2010 Aug 4;8(2):113–121. doi: 10.1016/S1672-0229(10)60012-5

Table 1.

Predicted miRNAs of tea

miRNA family EST ID miRBase best hit ID Mature sequencea ML MN Ab
miR164*** CV013669 osa-miR164e UGGAGAAGCAGGGCACGUGAG 21 2 3’
miR169*** GE650220 osa-miR169n AAGCCAAGAAUGACUUGCCa 21 3 5’
miR1846* DN976181 osa-miR1846-5p AUUGAGGAGGCCGGGaCuUCaU 22 3 5’
miR1863* GH623864 osa-miR1863 AGCUCUGAUACCAUaUUAGAaUAa 24 3 5’

miRNA family Precursor miRNAc
PL (nt) A (%) C (%) G (%) U (%) A+U (%) G+C (%) MFE
Start End

miR164*** 309 376 68 28 20 28 23.5 51.5 48.53 20
miR169*** 442 492 51 22 18 25 35.3 56.9 43.14 7.8
miR1846* 11 228 218 28 12 20 39.5 67.9 32.11 40.5
miR1863* 377 448 72 33 8.3 25 35.3 66.7 33.33 19.2
*

unique miRNA family registered in only one plant species in miRBase

***

highly conserved miRNA family registered in more than two plant species in miRBase.

a

Mature sequence of predicted miRNA. Lowercase represents the mismatch with the known miRNA sequence hit.

b

Location of the mature miRNA in the arm of the precursor sequence.

c

Location of the precursor sequence in the EST. ML, length of the mature miRNA sequence; MN, number of mismatches; PL, length of the precursor; A, C, G, U (%), adenine, guanine, cytosine, uracil nucleotide composition in the precursor miRNA; MFE, minimum free energy.