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. 2016 Oct 7;6:34719. doi: 10.1038/srep34719

Figure 2. JAK2 deficiency leads to increased mobilisation of fatty acids from the periphery and hepatic de novo lipogenesis.

Figure 2

(a) WAT/body weight ratio of perigonadal fat tissue indicating lipid mobilisation at indicated time points (n ≥ 4/genotype). Representative Western blot analysis of whole liver homogenates and Western blot quantification of CD36 from 12-week-old animals. HSC70 is shown as loading control (n = 3/genotype). Scans of blots are presented in Supplementary Fig. S8. At 12 weeks of age, FFA serum levels, which were determined using an enzymatic test, were elevated in all mice lacking JAK2 (n ≥ 5/genotype). Serum TG levels were measured at 12 weeks of age (n ≥ 4/genotype). (b) Representative Western blot analysis of whole liver homogenates from 12-week-old animals. As a loading control HSC70 is shown (n = 3/genotype). (c) Western blot quantification of total FAS, SCD1, PPARγ and PPARα. Scans of blots are presented in Supplementary Fig. S8. (d) Hepatic total FA and TG amounts were measured at 12 weeks of age (n ≥ 6/genotype). Ratio of saturated/unsaturated hepatic FAs at 12 weeks of age (n ≥ 8/genotype). (e) Detailed profiling of steatotic livers using gas chromatography–mass spectrometry (GC-MS) showed elevated unsaturated FAs at 12 weeks of age (n ≥ 8/genotype). *p < 0.05, **p < 0.01 and ***p < 0.001.