Figure 1. AML1/ETO regulates genes involved in cellular migration and adhesion.

(A) AML1/ETO protein levels in EML-AE clones used in this study were compared to those in patient-derived cell lines Kasumi-1 and SKNO-1 by Western blotting with an anti-ETO antibody. Sample loading was controlled by detection of Vinculin. (B) Kinetics of myeloid differentiation as measured by FACS analysis of cKit, Sca-1, Mac-1 and Gr-1 surface in untreated (0 days), atRA (3^rd day of treatment) and GM-CSF (8^th day of treatment) treated EML-EV, EML-AE14 and EML-AE22 cells. (C) Ingenuity Pathway Analysis (IPA) classification of functions enriched in the list of genes regulated in EML-AE22 cells compared to EML-EV cells identified by RNA-seq. (D) BloodSpot plots showing the expression data of public adhesion and migration signatures in AML subtypes and normal HSC/MPP cells. For each signature, the mean expression values for all samples in all datasets were computed and reported as dots in y-axis. Averaged values represented the expression of a signature for each sample. Statistical analysis was performed on the distribution of these values between the AML t(8; 21) dataset and the normal HSC dataset.