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. 2016 Oct 7;6:35008. doi: 10.1038/srep35008

Figure 7. RT-qPCR analysis of eight randomly selected genes to confirm expression patterns indicated by sequencing.

Figure 7

Three technical replicates were performed for each of the three biological replicates. The height of each bar chart represents the mean average of sample-specific 2−ΔΔCt values. “1” represents the egg stage, “2” represents the larval stage, “3” represents the pupal stage, and “4” represents the adult stage.