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. 2016 Aug 2;186(2):266–276. doi: 10.1111/cei.12839

Figure 2.

Figure 2

Differential expression of porcine inflammation‐related genes in the postmortem colonic explants harvested from the mature pigs and subjected to incubation at 3 h in the absence (a) or presence of lipopolysaccharide (LPS) (b). Sections of postmortem colonic tissue from mature pigs were processed and incubated in 1 ml Dulbecco's modified Eagle's medium (DMEM) containing 10 μg/ml LPS in a humidified cell culture incubator maintained at 37°C with 5% CO2 for 3 h. The abundance of gene transcripts was measured using a customized quantitative real‐time polymerase chain reaction (PCR) array and the gene expression value is presented relative to the tissue harvested at 0 h time‐point.