Fig. 2.

IGF-1R interaction with EGFR increases with cetuximab treatment in resistant cells. a Co-immunoprecipitation (IP) of EGFR and IGF-1Rβ in resistant cell lysates with increasing time of exposure to cetuximab. Reciprocal immunopreciptations with either EGFR or IGF1R are presented in right and left panels respectively. b As in A, but for sensitive cell lysates. c EGFR western blot in input and supernatant (sup) after IP with anti-EGFR in resistant cell lysate. 5 % of each sample is loaded. d as in C but for anti-IGF1R in resistant cell lysate