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. 2016 Oct 7;10:230. doi: 10.3389/fncel.2016.00230

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Copyright © 2016 Vastagh, Rodolosse, Solymosi and Liposits.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of differential gene expression data by quantitative real-time PCR. (A) List of genes (FC > 1.6; FDR < 0.05) selected for validation of their differential expression by qPCR. The qPCR confirmed the differential expression of 8 genes. RQ, relative quantity (B) The correlation coefficient (Pearson's r = −0.8445) indicates the quantitative correlation between the log₂ transformed and normalized spot intensity values of microarray hybridizations and the cycle threshold (Ct) value of the qPCR investigations. X-axis, cycle threshold value; Y-axis, normalized microarray expression values.