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. 2016 Oct 7;6:34925. doi: 10.1038/srep34925

Figure 1. Nutrient reduction promotes lasR mutant emergence during in vitro evolution of WT PAO1.

Figure 1

(a) Long-term evolution assays were performed in gradient QS medium with or without pre-added QS signal 3OC12-HSL to detect the proportions of the strains that failed to grow in M9-adenosine medium (Y-axis), and the cell density was measured before the starting of next cycle (b). The evolutionary screening was also performed in gradient MOPS-buffered LB broth. The experiments were designed as routinely shaking (c) and biofilm-related static cultures (d). FS, full-strength LB broth; 1/2-S, 1/2-strength LB broth; 1/4-S, 1/4-strength LB broth; 1/8-S, 1/8-strength LB broth; 1/16-S, 1/16-strength LB broth.